- Loss of Heterozygosity on Chromosome 15q15 Near Thrombospondin-1 Gene in Breast Carcinomas.
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Jeana Kim, Kyoung Mee Kim, Heejeong Lee, Kyungji Lee, Mun Gan Rhyu, Anhi Lee, Seok Jin Kang, Kyo Young Lee
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Korean J Pathol. 2009;43(3):221-230.
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DOI: https://doi.org/10.4132/KoreanJPathol.2009.43.3.221
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 Abstract
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- BACKGROUND
Chromosome 15q15 near the thrombospondin-1 (THBS-1) gene may be associated with tumor progression and metastasis. To clarify the potential role of the15q15 region in progression of breast carcinoma, we investigated the loss of heterozygosity (LOH) and the microsatellite instability (MSI) status of chromosome 15q15. Methods : LOH and MSI were detected in 84 breast carcinoma specimens using PCR-based microsatellite analysis with three microsatellite markers.
METHODS
LOH and MSI were detected in 84 breast carcinoma specimens using PCR-based microsatellite analysis with three microsatellite markers.
RESULTS
Of 77 breast carcinomas containing the heterozygous alleles, 25 (32%) showed LOH in at least one microsatellite marker. Partial LOH and total LOH were detected in 14 (18.27%) and 11 (14.3%) cases. The total LOH were inversely correlated with node metastasis. A single LOH at D15S514 was inversely correlated with nuclear grade and a single LOH at the D15S129 allele was associated with increased expression of the THBS-1 gene. MSI-positive breast carcinomas detected in 14 (17%) cases showed no correlation with any clinicopathologic feature.
CONCLUSIONS
These results indicate that loss of the chromosome 15q15 region delays the progression of breast carcinoma because the magnitude of LOH is large and involves the THBS-1 gene and additional genetic elements. The genes located on chromosome 15q15 probably play a tissue-type-dependent role in malignant growth of the tumor.
- Borderline Clear Cell Adenofibromatous Tumors of the Ovary: Two Case Reports.
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Heejeong Lee, Tae Jung Kim, Jeana Kim, Eun Joo Seo, Kyo Young Lee
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Korean J Pathol. 2007;41(6):420-423.
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Abstract
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- Borderline clear cell adenofibromatous tumors are rare with only 26 cases reported in the English literature. Five of these cases exhibited microinvasion and 4 demonstrated intraepithelial carcinoma. We report 2 cases, one typical case and the other with microinvasion. The histological findings revealed widely spaced and focally crowded, variably-sized atypical glands or tubules lined by clear, eosinophilic or hobnail cells set in a dense fibrous stroma.
One of the two cases had small solid nests or single cells in the stroma around the proliferative glands less than 1 mm in length that was considered to be a microinvasion.
- Correlations between the Expression of c-Abl, c-Kit, Platelet-derived Growth Factor Receptor (PDGFR)-alpha and PDGFR-beta and Survival in Patients with Ovarian Cancer.
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Heejeong Lee, Keun Ho Lee, Kyo young Lee, Chang Suk Kang
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Korean J Pathol. 2006;40(3):210-216.
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Abstract
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- BACKGROUND
:Protein tyrosine kinases (PTKs) such as c-Abl, c-KIT, PDGFR-alpha and PDGFR-bata are key proteins in the regulation of cell growth. In this study, we evaluated the correlations between the expression of c-Abl, c-KIT, PDGFR-alpha and PDGFR-beta and the survival of patients with ovarian cancer.
METHODS
We performed the immunohistochemistry for 102 patients with ovarian cancer and we retrospectively reviewed the overall and disease free survival and also the response to platinum-based chemotherapy in those patients.
RESULTS
The short disease free survival rate was significantly associated with the increased expression of PDGFR-alpha (p=0.0459). The short overall survival time in patients with advanced (stage III and IV) ovarian cancer was associated with the overexpression of c-Abl (p=0.0268) and the reduced expression of c-KIT (p=0.0307). On multivariate analysis, the tumor stage and c-Abl maintained their prognostic influence. Meanwhile, none of the four PTK expression patterns predicted the response to the platinum-based chemotherapy.
CONCLUSIONS
Our data suggest that for patients with advanced ovarian cancer, the overexpression of c-Abl and the reduced expression of c-KIT might be used as poor prognostic factors for overall survival. It is further noteworthy that the tumor stage and c-Abl may be useful in predicting the patients' survival. Although any of the four PTKs could not predict the response to platinum chemotherapy, the expression of the kinases targeted by tyrosine kinase inhibitor suggests the potential usefulness of imatinib mesylate for the treatment of ovarian cancer.
- Expressions of Cyclin E-pathway Proteins (cyclinE, cdk2, p21, p27, p57) and Their Prognostic Significance in Non-small Cell Lung Carcinomas.
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Ji Han Jung, Gyeongsin Park, Myung Ah Lee, Jae Ho Byun, Chan Kwon Jung, Heejeong Lee, Kyo Young Lee, Sang In Shim, Chang Suk Kang
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Korean J Pathol. 2006;40(1):24-31.
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Abstract
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- BACKGROUND
The aberrant expression of cyclins, cdk and cdk inhibitor has been shown to be involved in oncogenic transformation. The aim of this study was to investigate the expression of the cyclin E-pathway proteins (cyclin E, cdk2, p21, p27, p57) in human non-small cell lung carcinomas (NSCLC) and also to evaluate the clinical significance of these expressions.
METHODS
A total of 203 consecutive patients with completely resected pathological stage I-III NSCLC were retrospectively reviewed. The expressions of cyclin E, cdk2, p21, p27 and, p57 was examined by performing immunohistochemistry with using the tissue microarray method.
RESULTS
In the total cases, the expression levels of cyclin E, cdk2, p21, p27 and p57 were 39.9% (81/203), 48.3% (98/203), 68.0% (138/203), 32.5% (66/203) and 2.7% (5/203), respectively. The overexpression of cyclin E and cdk2 was significantly and inversely correlated with the histologic differentiation in the adenocarcinoma (p<0.05), but not in the squamous cell carcinoma. Among the clinicopathologic factors, the stage and lymph node metastasis were associated with overall survival (p<0.05). Among these proteins, the negative expression of p21 was significantly correlated with a shortened survival rate (p<0.05).
CONCLUSIONS
These data suggest that the overexpression of cyclin E and cdk2 and the loss of p21 and p27 are associated with tumor progression in NSCLC. The aberrant expression of p21 is correlated with a poor prognosis. Therefore the immunohistochemical analysis of this protein as well as the clinical stage and, lymph node metastasis may be useful tools for evaluating the prognosis of NSCLC patients.
- Metastasizing Atypical Chondroid Lesion: A Case Report.
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Heejeong Lee, Jinyoung Yoo, Seok Jin Kang, Byung Kee Kim
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Korean J Pathol. 2003;37(1):62-65.
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- We recently experienced an atypical chondroid lesion metastatic to the lung. Examined under the microscope, the excised nodules displayed abundant amounts of chondroid tissue and necrosis with dystrophic calcification. The calcification had a characteristic "chicken wire" pattern.
The chondroblasts contained one or two round to oval, focally indented nuclei with inconspicuous nucleoli.
Although some enlarged nuclei with mild to moderate pleomorphism were present, significant nuclear atypia was lacking. Mitotic figures were scarce (1/10 high power field). Scattered multinucleated osteoclast-type giant cells were observed among the chondroblasts. Thus, the possibility of chondroblastoma was considered first. Metastasis of histologically benign chondroblastoma is a rare event, but well documented. The pulmonary metastatic nodules were described as ceasing to grow in some cases; and a significant number of patients were free of tumors following removal of the metastatic nodules. These findings suggest that the metastasis may represent a simple transport phenomenon. However, there are, as yet, no histologic parameters that help determine whether these metastases are to cease their growth or will progress to kill the host.
- Primary Leiomyosarcoma of Adrenal Gland: A Case Report.
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Heejeong Lee, Jinyoung Yoo, Seok Jin Kang, Byung Kee Kim
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Korean J Pathol. 2002;36(3):191-194.
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- Primary mesenchymal neoplasm of the adrenal gland is very rare. Recently we experienced a case of leiomyosarcoma of the adrenal gland in a 47-year-old female patient. The resected adrenal gland showed a large lobulated mass, which replaced the entire gland. The cut surface was firm and whitish gray with foci that showed hemorrhage and necrosis.
Histologically, the tumor was composed of intersecting fascicles of pleomorphic spindle cells with numerous giant cells and mitotic figures. Some of the tumor cells showed elongated nuclei. Immunohistochemical studies were strongly positive for vimentin and smooth muscle actin. Cytokeratin, desmin, alpha-1-antitrypsin and lysozyme were all negative.
To the best of our knowledge, this is the first case reported in Korea.
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